| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Rothia aeria | ACCGAGCTTGTGGTGAAGG | CGCGATCACCATAGGCCAT | 59.93 | 60.00 | 259 | 131 |
100.00%
|
100.00%
|
| Primer 2 | Rothia aeria | TGCGGTCCTTGCAAGCA | TGTGACTCGTTGAGCGCA | 59.84 | 59.58 | 197 | 120 |
100.00%
|
100.00%
|
| Primer 3 | Rothia aeria | CTTGCAAGCAGTTCTCGCC | TGTGACTCGTTGAGCGCA | 59.79 | 59.58 | 190 | 120 |
100.00%
|
100.00%
|
| Primer 4 | Rothia aeria | TTGCAAGCAGTTCTCGCC | TGTGACTCGTTGAGCGCA | 58.66 | 59.58 | 189 | 120 |
100.00%
|
100.00%
|
| Primer 5 | Rothia aeria | AGCTTTCCGGTAAGGTGCC | AGCCTGTGAAACTGCGCA | 60.00 | 60.20 | 199 | 120 |
100.00%
|
100.00%
|
| Primer 6 | Rothia aeria | TCCGGTAAGGTGCCTGAGT | AGCCTGTGAAACTGCGCA | 60.23 | 60.20 | 194 | 120 |
100.00%
|
100.00%
|
| Primer 7 | Rothia aeria | TGGCACGCAGGAAACGTA | GTGGCCTTGGAGAGCACAT | 59.58 | 60.00 | 257 | 115 |
100.00%
|
100.00%
|
| Primer 8 | Rothia aeria | TGGCACGCAGGAAACGTA | ACCTTCTCGTGTGGCCTTG | 59.58 | 59.93 | 267 | 115 |
100.00%
|
100.00%
|
| Primer 9 | Rothia aeria | TGGCACGCAGGAAACGTA | TTCTCGTGTGGCCTTGGAG | 59.58 | 59.63 | 264 | 115 |
100.00%
|
100.00%
|
| Primer 10 | Rothia aeria | TGGCACGCAGGAAACGTA | CTTCTCGTGTGGCCTTGGA | 59.58 | 59.63 | 265 | 115 |
100.00%
|
100.00%
|